Expression of bovine beta-1,4-galactosyltransferase cDNA in COS-7 cells.
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چکیده
منابع مشابه
Cloning and high level expression of bovine interferon gamma gene in eukaryotic cells (COS-7)
Interferon gamma (IFN-γ) is one of the key cytokines in defining T helper 1 lymphocyte immuneresponses. In this study, the bovine IFN-γ gene was cloned from spleen tissue RNA using the reversetranscription-polymerase chain reaction (RT-PCR). IFN-γ cDNA was sub-cloned and expressed inmammalian expression plasmid (pcDNA3.1(+)) under the control of the human cytomegalovirus (CMV)promoter. The pred...
متن کاملcloning and high level expression of bovine interferon gamma gene in eukaryotic cells (cos-7)
interferon gamma (ifn-γ) is one of the key cytokines in defining t helper 1 lymphocyte immuneresponses. in this study, the bovine ifn-γ gene was cloned from spleen tissue rna using the reversetranscription-polymerase chain reaction (rt-pcr). ifn-γ cdna was sub-cloned and expressed inmammalian expression plasmid (pcdna3.1(+)) under the control of the human cytomegalovirus (cmv)promoter. the pred...
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Lactosylceramide synthase is an enzyme that catalyzes the transfer of galactose from UDP-Gal to glucosylceramide, and thus participates in the biosynthesis of most glycosphingolipids in mammals. We purified this enzyme over 61,000-fold to near homogeneity with a 29. 7% yield from rat brain membrane fractions. The isolation procedure included solubilization with Triton X-100, affinity chromatogr...
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Hepatitis B virus (HBV) is a serious global health problem. The development of a safe and effective vaccinewould help infection prevention. Previous hepatitis B vaccine production involved the isolation of the noninfectious particle from chronic HBV carriers. DNA recombinant technology has been used for vaccineproduction without having been contaminated with blood-born infectious ag...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.15.5733